Special Pathogens Research Network Limited, Uganda

 MIRU-VNTR genotyping workshop

Mycobacteium Interspaced Repetitive Unit Variable Numbers of Tanderm Repeats MIRU-VNTR genotyping workshop at the Department of Microbiology, College of Health Sciences, Makerere University Kampala, Uganda from June 27 to July1, 2016


Six days MTB training workshop [June 27 to July 1, 2016 ] in Kampala Uganda


9.0 –11am



1.0 -1.50pm




June 27

Reporting , Onsite registration

Tour of the College facilities, Speeches, remarks,

Breaking the ice

 Key officers of Spparenet Ltd and CHS Facilitators



Key-note address:

Competence  in MTB diagnosis

Dr Agwu Ezera



Round table discussion on application of workshop

skills To participants career development

Dr Agwu Ezera



June 28

Review of phenotypic methods in Mycobacteria disease diagnosis

* Microscopy and staining methods in Mycobacteria detection    (Dr Agwu Ezera)


Microscopy in Mycobacteria detection (Lab practicals)

Culture of TB [Alternative to practicals] Quality Assurance

(CHS staff)



Spoligotyping and DNA finger printing
(CHS staff)



June 29

Molecular detection of Mycobacteria species

[DNA Finger-printing and MIRU-VNTR] Lecture


Sample collection, processing and storage for DNA Finger-printing and MIRU-VNTR. Quality Assurance

(Dr Agwu Ezera) 


Primer design and selection, Probe preparation and optimization

(CHS staff)


June 30

Hands on MIRU-VNTR technology


Hands on MIRU-VNTR technology


Hands on MIRU-VNTR technology


July 1, 2016


Hands on MIRU-VNTR technology

(CHS staff)


Hands on MIRU-VNTR technology

(CHS staff)


Hands on MIRU-VNTR technology

(CHS staff)



June 28

Closing remarks,

Award of certificates

Session closing





Facilitators: Dr Agwu Ezera, Prof Moses Joloba, Dr Henry Kajumbula, Dr Samuel Kyobe and others...

Request for details about this workshop by sending mails  to info@spparenet.us or you can click here...

See Poster for 2016 MIRU-VNTR genotyping workshop here...

See the poster for the 2015 workshop here...



The pictures of our past MIRU-VNTR workshops are shown in the photo gallery below. It shows strategic defining morments for skill acquisition, lecture, question and answer, career counselling, discusion of matters arrising, tea break, award of certificates and so on. Click the pictures to enlarg Happy viewing

Overall mission statement of our workshops

To provide high quality, value driven, patient centred services based on best practice, research and evidence which are sensitive and responsive to the needs of the population, service users and providers.

Expected competencies at the end of the workshop

a.       Operate a quality management system to integrate the organisation, procedures, processes and resources of a Department;

b.      Set quality objectives and plans in order to implement this quality policy;

c.       Ensure that all staff is familiar with the quality policy, all procedures relevant to their work and the quality manual;

d.      Commit to the health, safety and welfare of all its staff; visitors to the Department’s laboratories will be treated with respect and due consideration will be given to their safety while on site;

e.       Commit to comply with relevant environmental legislation;

f.       Uphold professional values and comply with good professional practice and conduct.

g.      Staff recruitment, induction, training, development and retention at all levels so as to provide a full and effective service to its users.

h.      The proper procurement and maintenance of such equipment and other resources as are needed for the provision of the service.

i.        The collection, transport and handling of all specimens to ensure the correct performance of laboratory examinations

j.        The use of examination procedures that will ensure the highest achievable quality of all tests performed.

k.      The reporting of examination results which are timely, confidential, accurate and clinically useful

The assessment of user satisfaction, in addition to internal audit and external quality assessment, in order to produce continual quality improvement